Fig. 3

Heat map and clustering representing most similar transcriptome profiles among Tuta absoluta developmental stages. The count matrix for all sequenced samples was used to calculate an Euclidian distance matrix, which was used for hierarchical sample clustering, according to the most similar transcriptome profile using the single linkage method, to generate a dendrogram and a heatmap correlating all sample expression profiles into colors, ranging from red (identical profiles) to green (most different). The developmental stages analyzed were egg (TA_EG); 1^st stage larvae (TA_LI_STG1); 2^nd stage larvae (TA_LI_LSTG2); 3^rd stage larvae (TA_LI_LSTG3); 4^th stage larvae (TA_LI_STG4); and adults (TA_AD)