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Fig. 4 | BMC Genomics

Fig. 4

From: De novo transcriptome assembly and analysis to identify potential gene targets for RNAi-mediated control of the tomato leafminer (Tuta absoluta)

Fig. 4

Comparison of relative gene expression based on RT-qPCR or RNA-seq among developmental stages. Based on the RNA-seq data, 23 contigs with significant differential expression between developmental stages (egg, larval and adults) were chosen to be validated by RT-qPCR. Relative expression of 4 genes (58512; 75835; 77615; and 81147; Additional file 5: Table S5) based on RT-qPCR is represented by whiskers-box plots with standard deviations, and RNA-seq data is represented by fold-differences. Whisker-box plots display average values from three biological replicates, and the box contain 50 % of the variation among samples, while the remaining 50 % are divided between the upper quartile (25 %) and the lower quartile (25 %), represented by error bars (whiskers). The other 18 genes are presented on Additional file 6: Figure S1

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BMC Genomics

ISSN: 1471-2164

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