Fig. 7
RNAi effects of 8 genes on Tuta absoluta 1st instar larvae fed in tomato leaflets. a. Relative expression of target genes in larvae, five days after fed on tomato leaflets that absorbed 10 μg of dsRNA from each gene target (592; 2594; 4623; 1360; and 4303), and a eGFP control, plus three genes with previous positive results (V-ATPase; AK; EcR; Camargo et al., unpublished method) all in triplicates. Gene expression is expressed in relation to eGFP-fed control. b. Average weight of larvae (n = 30) under the same conditions as above. Bars represent standard deviation; ‘*” or ‘**”represent significant at P < 0.05 or P < 0.01, respectively by T-student test. Gene expression used Rpl5 as gene reference