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Fig. 6 | BMC Genomics

Fig. 6

From: Transcriptional analysis of phloem-associated cells of potato

Fig. 6

Select genes that are differentially expressed in petioles of potato in response to photoperiod (a). Mean reads from RNA-seq data are presented for both petiole- and stem-PAC and for LD/SD petioles. Real time RT-PCR was used to verify the RNA-seq results for SD-induced (b) or LD-induced (c) genes. Each gene in panels b and c is designated by its 5-digit PGSC identifier (in bold, a). Relative levels of their transcripts were quantified using total RNA extracted from petiole samples harvested from plants grown under long (open bars) or short (shaded bars) days. Short-day plants were harvested after 10day of SD conditions (8 h light, 16 h dark). Quantitative real-time RT-PCR with gene-specific primers was used to calculate the relative amounts of RNA for each gene. The expression of each gene was calculated as the 2-ΔCt value and normalized to the endogenous reference gene, StAct8. Standard errors of the means of three biological replicates are shown with 1 and 3 asterisks indicating significant differences (p < 0.05, p < 0.001, respectively) using a Student’s t test

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