Fig. 4
From: EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus
SDS–PAGE analysis of recombinant His-EDDIE-AMPs expressed in E. coli BL21 (DE3). Lane M: the molecular weight markers; Lane 1: crude cells extracts of uninduced E. coli BL21 containing pET-His-EDDIE-AMP; Lane 2–18: crude cells extracts after 5 h past the induction with IPTG of E. coli BL21 containing pET-His-EDDIE-AMP. Arrows indicate the bands of fusion proteins of about 20 kDa