Fig. 3

N50 of assembly results for different library sizes. N50 values for four different library sizes (300 bp, 400 bp, 500 bp and 600 bp) for E. coli DH1 and S. Parasanguinis FW213 are shown together with their standard error of the mean for different depths of reads. a and b are N50 from Group A [PE] and c and d are N50 from Group A [SE] which are results from all reads assembled as paired-end reads and single end reads, respectively. e and f illustrate the results from Group A [PE + SE] which assembled all the non-overlapped paired-end reads and merged reads altogether. From A and B, we observed that larger library size tended to produce larger N50 values when assembled as paired-end sequence but not when assembled as single-end sequence (c and d). These increases in values were attributed to the utilization of pair-end information