Table 3 Validation of deregulated miRNAs and correlating target mRNAs in HN2092 after radiochemotherapy treatment (analyzed with Agilent microarrays and TaqMan single qRT-PCR assays)
miRNA | Array | qRT-PCR | mRNA | Array | qRT-PCR |
|---|---|---|---|---|---|
FC (p value) | FC (p value) | FC (p value) | FC (p value) | ||
miR-93-5p | 0.96 (0.001) | 0.84 (<0.001) | CCDC88C | 1.44 (<0.001) | 1.04 (0.890) |
miR-99b-5p | 1.13 (<0.001) | 1.10 (0.502) | ACTR1B | 0.62 (<0.001) | 0.61 (0.012) |
miR-181a-5p | 1.38 (<0.001) | 1.37 (0.001) | – | – | – |
miR-183-5p | 1.18 (<0.001) | 1.18 (0.071) | ASNS | 0.52 (<0.001) | 0.47 (0.017) |
| Â | Â | Â | IDH2 | 0.63 (<0.001) | 0.62 (0.045) |
| Â | Â | Â | HSPA1B | 0.52 (0.022) | 0.81 (0.167) |
miR-186-5p | 1.15 (0.083) | 1.10 (0.147) | FASN | 0.66 (<0.001) | 0.66 (0.007) |
miR-197-3p | 1.17 (0.025) | 1.02 (0.273) | FASN | 0.66 (<0.001) | 0.66 (0.007) |
| Â | Â | Â | HSPA1B | 0.52 (0.022) | 0.81 (0.167) |
miR-222-3p | 1.10 (0.006) | 0.95 (0.057) | FASN | 0.66 (<0.001) | 0.66 (0.007) |
miR-320a | 1.14 (<0.001) | 1.07 (0.425) | HES6 | 0.65 (<0.001) | 0.52 (0.004) |