Table 4 Primer sequences used in this study
From: Transcriptomic changes of Legionella pneumophila in water
Name | Purpose | Sequence (5’➔3’)a |
|---|---|---|
16Â s_QF | 16Â s rRNA qPCR | AGAGATGCATTAGTGCCTTCGGGA |
16Â s_QR | 16Â s rRNA qPCR | ACTAAGGATAAGGGTTGCGCTCGT |
25_QF | lpg0025 qPCR | ATTCCCATCGCCATTTAGAG |
25_QR | lpg0025 qPCR | CAACCCGAGAGGTAACTAATAC |
586_QF | lpg0586 qPCR | GTGGCGTTCCAGTTTGT |
586_QR | lpg0586 qPCR | CTGTCCAGGCAGCATAAC |
846_QF | lpg0846 qPCR | GGTAGAAGGCGATGGTTATC |
846_QR | lpg0846 qPCR | GCCTTCCGGTGGTAATAAA |
890_QF | lpg0890 qPCR | CCTTCCAATCCCATGCTAAAG |
890_QR | lpg0890 qPCR | GTCAAATCCGAGTTCAAGAGG |
1206_QF | lpg1206 qPCR | GCGTCATGAGGATTCTATTCG |
1206_QR | lpg1206 qPCR | GGCCTGTAAATCGTATCAGAC |
1284_QF | lpg1284 qPCR | GTTTATCTCAGAGCGGCAAG |
1284_QR | lpg1284 qPCR | GACATCCTCCAAAGGCTTATC |
1659_QF | lpg1659 qPCR | CGGTCACTCTTTGGTATATGTC |
1659_QR | lpg1659 qPCR | CTGATTGACTGGATCGAACATC |
2316_QF | lpg2316 qPCR | GCCATGTAGCAGAGGAAATC |
2316_QR | lpg2316 qPCR | CTTTATCCACGCCCTGATTG |
2487_QF | lpg2487 qPCR | TCTGTATCTCGGAGCCTATG |
2487_QR | lpg2487 qPCR | GTGGCCTAAACCTGATCTTG |
2524_QF | lpg2524 qPCR | CGCCTGGTATAAAGAACTGC |
2524_QR | lpg2524 qPCR | GAGGCGAAGGTAACCATTTC |
bdhA_UpF | Mutant | AGTTCAATACAATCCTTGGTCGC |
bdhA_UpR | Mutant | CACGAATTCCTTTTACTATCCTTGTCATTG |
bdhA_GnF | Mutant | CGCGAATTCAACGGCATGATGAACCTGAAT |
bdhA_GnR | Mutant | CACTCTAGATTAGGTGGCGGTACTTGGGTC |
bdhA_DownF | Mutant | CGCTCTAGAACAACCATGACTCGAACTAAAAAATCT |
bdhA_DownR | Mutant | CTTTTGAAGACAATTCCGTTTCAT |
Com_bdhA_F | Complement | CGCGAGCTCGACAAGGATAGTAAAAGAATGAAACTGAAG |
Com_bdhA_R | Complement | CGCTCTAGATCATGGTTGTTTACTCCATGAACC |
PromF | Complement | CGTATAATGTGTGGAATTGTGAG |
pXDC39-F | GFP assay | GCTTCCACAGCAATGGCATCC |
GFP-R | GFP assay | TGTCGACAGGTAATGGTTGTC |
GFP_bdhA_F | GFP assay | CGCTCTAGACATAGGGATATCAACCACTACG |
GFP_bdhA_R | GFP assay | CGCTCTAGATCTTTTACTATCCTTGTCATTG |