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Fig. 2 | BMC Genomics

Fig. 2

From: Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin (Lupinus angustifolius)

Fig. 2

Validation of simple PCR-based SNP markers linked to the R gene PhtjR conferring phomopsis stem blight disease resistance on all 27 historical and current cultivars of Lupinus angustifolius released in Australia by high resolution melting (HRM) on LightScanner. SNP marker DAFWA6277 (left) was confirmed as diagnostic for the PhtjR gene, as the three cultivars (Wonga, Tanjil and Barlock) showed the resistance marker allele (melting curves in blue), while all the other 23 cultivar not possessing the R gene has the susceptible marker allele (melting curves in red). In contrast, SNP marker DAFWA3123 (right) was confirmed as non-diagnostic, since six cultivars (TableĀ 7) without the R gene had the resistance marker allele (melting curves in blue). Detailed records of genotypes for 27 cultivars of these two markers are presented in TableĀ 7

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