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Fig. 3 | BMC Genomics

Fig. 3

From: Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin (Lupinus angustifolius)

Fig. 3

Validation of InDel markers arising from marker mining on genome sequence assembly scaffold84773 linked to the R gene PhtjR conferring phomopsis stem blight disease resistance on all 27 historical and current cultivars of Lupinus angustifolius by polyacrylamide electrophoresis gels. The 27 cultivars are: Uniwhite (Lane 1), Uniharvest (Lane 2), Unicrop (Lane 3), Marri (Lane 4), Illyarrie (Lane 5), Yandee (Lane 6), Chittick (Lane 7), Danja (Lane 8), Geebung (Lane 9), Gungurru (Lane 10), Yorrel (Lane 11), Warrah (Lane 12), Merrit (Lane 13), Myallie (Lane 14), Kalya (Lane 15), Wonga (Lane 16), Belara (Lane 17), Tallerack (Lane 18), Tanjil (Lane 19), Moonah (Lane 20), Quilinock (Lane 21), Jindalee (Lane 22), Mandelup (Lane 23), Coromup (Lane 24), Jenabillup (Lane 25), Gunyidi (Lane 26) and Barlock (Lane 27). Disease phenotypes of the cultivars are presented as “S” (susceptible) or “R” (resistant) in blue letters. Marker “InDel10” was confirmed as diagnostic for the PhtjR gene, since it showed the marker genotypes consistent with PSB phenotypes on all cultivars. In comparison, marker “InDel 66” was confirmed non-diagnostic, since eight cultivars (arrowed in red) without the R gene had the resistance marker allele (“false positives”)

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