Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin ( Lupinus angustifolius )

Table 9 Conversion of SNP markers and InDel markers arising from marker mining on scaffold84773 into sequence-specific PCR markers in Lupinus angustifolius

Marker name	Primers	Primer sequence (5′-3′)
SNP 20	SNP20F	GTCCCTGCCATTATTAATAGTTACT
SNP 20	SNP20R	CATCATGAGTCAATTTACCACTTA
SNP 25	SNP25F	GTCACTAATTTTATCTTTGCAAGA
SNP 25	SNP25R	GATCATAAGAATAATAATAATAATTTGGT
SNP 250	SNP250F	GACTTAGTAATGTGCAACAAGAG
SNP 250	SNP250R	CTGACACTACAGGTTCGCCT
SNP 263	SNP263F	GGAACATTGTGATTCAGTCACC
SNP 263	SNP263R	GATAGGTTTGTTGCAATAAGCG
SNP264	SNP264F	GTTTCTTAGTTGCATAGTTGCAA
SNP264	SNP264R	CAAAACATTCATAAGTAACAAGG
SNP271	SNP271F	CGACACCATCTGATATATGAAAATAA
SNP271	SNP271R	ACCGGAAATCTGTGTTTTTC
InDel2	InDel2F	GATAAAGTATATCTAAATTATGTTTGC
InDel2	InDel2R	CTATATTTTGTATCAATTATAACAAATT
InDel10	InDel10F	GTTAAGTGGTAAATTGACTCATG
InDel10	InDel10R	GTTTTRCATTCTTGCAAAGATAAAATTAG
InDel28	InDel28F	CTACAATAGCCACACAAATAG
InDel28	InDel28R	GTTTAGATGGCCMTGTGC
InDel66	InDel66F	CTTCTGAGTTGGACCATAAAC
InDel66	InDel66R	ACTCACATTTACAGAACTTTAACT

ISSN: 1471-2164