Fig. 3

Combination of fluconazole and CR, CAS or ZYM exhibits a synergistic growth-inhibitory effect on yeast cells. Cell growth assays were carried out in 96-well microtiter plates, preparing two-fold serial dilutions of fluconazole (FZ) to give concentrations ranging from 128 to 16 μg/ml in a final volume of 150 μl of YEPD, either including a constant concentration of CR (30 μg/ml, FZ + CR), CAS (15 ng/ml, FZ + CAS) or ZYM (64 U/ml, FZ + ZYM) or not including the cell wall stress (FZ). Cultures containing only CR, CAS or ZYM were also included as growth control. Representative experiments for each condition are shown