Fig. 4

MLP1 induction by cell wall stress is reduced in some mutants belonging to the CWM group. Expression of pMLP1-LacZ was measured in wild-type (BY4741) and scp160∆, def1∆, sph1∆, ies6∆ mutants growing during 3 h at 30 °C either in the absence or in the presence of CR (30 μg/ml), CAS (15 ng/ml) or ZYM (0.8 U/ml). Relative β-galactosidase activity respect to the wild-type strain (100 % of activity) for each condition is presented. Three independent experiments were carried out to calculate means and standard deviations