Fig. 2

Double RNAi led to additive but not synergistic effects. a–l Double RNAi treatments were performed and the number of surviving animals at different days post injection was documented (see values in Additional file 1: Table S4). From these values, the number of surviving animals of the respective single treatments (m) was subtracted, such that higher lethality in the double treatment is indicated by negative values in the panels. For every target gene, ten different pairwise combinations were injected with a total dsRNA concentration of 0.5 ng/μl and compared to the single injections (0.5 ng/ul total concentration). m Single injection of the 11 RNAi target genes were performed with a dsRNA concentration of 0.5 ng/μl