Fig. 3

GO term clustering reveals the proteasome as efficient target. a All 11 top RNAi target genes have Drosophila orthologs and overall, their protein sequence identity with other species is high. Only Cact, Inr-a and Gw show a low degree of sequence conservation. Dm Drosophila melanogaster, Am Apis mellifera, Aa Aedes aegypti, Ap Acyrthosiphon pisum. b GO term clustering of the top 40 RNAi target genes revealed ten clusters with enriched biological processes. Searching for genes that share the respective GO term combinations identified additional RNAi target genes. For cluster 1, seven novel genes with the respective combination were found and six of them (86 %) turned out to be highly lethal at day 11 (d11) after injection. Cluster 1, 2 and 7 showed the highest predictive power. c The GO terms of the most predictive clusters are shown. Count is the number of genes annotated with a given term. GOTERM_BP_FAT are annotations with respect to biological processes (see Additional file 2: Figure S4 for all clusters)