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Fig. 3 | BMC Genomics

Fig. 3

From: Malat1 as an evolutionarily conserved lncRNA, plays a positive role in regulating proliferation and maintaining undifferentiated status of early-stage hematopoietic cells

Fig. 3

Expression analysis of Malat1 gene in hematopoietic cells. a Northern blot showed the expression level of full length Malat1 in ATRA induced EML cells and Epro cells, upper panel: total RNAs from different induction stages of EML cells as designated in the figure. Lower panel: Semi-quantitative analysis of relative expression level for Malat1 gene in indicated EML and Epro cells; b Northern blot showed the expression level of full length Malat1 in different populations of mouse bone marrow as indicated in the figure. Upper panel: total RNA from LRH+, LRH-/(LRH) and LRH-/(LRB) were detected with Malat1probe. Lower panel: semi-quantitative analysis of relative expression level for Malat1 gene in these populations. c Northern blot showed the expression level for Malat1 gene in multiple tissues of mouse. Upper panel: total RNA from mouse tissues as showed in the figure was detected. Lower panel: semi-quantitative analysis of relative expression level for Malat1 gene in mouse tissues. 225 bp 5’ Malat1 DNA was used as the probe to detect the Malat1 expression, a ~6.9 kb Malat1 RNA was labeled in the figures, the data was been normalized using β-actin, for further details see material and methods

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BMC Genomics

ISSN: 1471-2164

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