Fig. 5

p53 bound to Malat1 promoter and inhibited its expression. a Over expression of p53 protein (around 2.7 fold up regulation) in EML cells down regulated Malat1 level with around 4 times, β-actin was used as a loading control, the means ± SD of three independent experiments are shown, P < 0.05 (over expression of P53 vs Mock control). b CHIP assay to detect the cells with over expression of p53 gene. Rabbit IgG was used as the negative control of CHIP, the means ± SD of three independent experiments are shown, P < 0.05 vs Mock control (co-immunoprecipitated with rabbit IgG). c Schematic drawing shows RNA polymerase II initiation complex binds to Malat1 promoter region, and p53 protein inhibits DNA binding activity of pol II therefore interferes the initiation complex assembly on transcription start site as indicated in the figure