Fig. 2

Mitochondrial OXPHOS genes respond differentially to treatments and assembly factors tend to express differently from nuclear genes encoding OXPHOS subunits. Expression profiles of OXPHOS genes are shown in heatmap representation in 32 RNA sequencing measurements of control and complex I-deficient patient cells. Panel a shows the mitochondrial-encoded OXPHOS genes. Panel b shows the nuclear-encoded OXPHOS genes. At the top of the figure, controls, patients, and compound incubations are indicated across the samples, where numbers 1 and 2 refer to the cell line. On the right assembly factors and subunits per complex are labeled by color. On the left the central cluster of OXPHOS subunit genes is indicated by a vertical bar. Genes were clustered using average linkage clustering with uncentered Pearson correlation as distance matrix. On the bottom the horizontal bar depicts expression values. A value of 1.0 (black) denotes median log-expression of the gene (see Methods), with green denoting higher and red denoting lower expression levels