Skip to main content
Fig. 2 | BMC Genomics

Fig. 2

From: Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq

Fig. 2

Male candidate gene expression in vivo. Whole mount in situ hybridisation for 4 male-biased candidate genes, on E6 UGS (UGS) from males and females. BMPR2 is more highly expressed in males (a, e) than in females (i, m). In over-stained sections, BMPR2 appears to be expressed in the testis cords (e). ZNF385b shows greater expression in males (b, f) than females (j, n), consistent with RNA-seq. In males it is expressed in the cords (f). NZP, a novel Z-protein, is expressed in males more highly than females (c, g versus k, o). It is also expressed in the cords of males (g). LAMA1 is also higher in males (d) than females (l) and is expressed in testis cords in males (h), with some weak expression in the juxta-cortical medulla in females (p). These results are consistent with RNA-seq data. Typically, 3 UGS from each sex were used for each probe, and these images are representative. A sense control probe did not show any staining for any of the candidate genes (data not shown)

Back to article page