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Fig. 3 | BMC Genomics

Fig. 3

From: Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq

Fig. 3

Female candidate genes expression in vivo. Whole mount in situ hybridisation for 3 female-biased candidate genes, on E6 UGS from males and females. CAPN5 is more highly expressed in female (a) than in males, where only low staining is observed (g, j). CAPN5 is also expressed in the adrenal glands in both females and males (a, g arrows). In over-stained sections CAPN5 appears to be expressed in the juxta-cortical medulla of the ovary (d). GPR56 shows strong expression in female gonads (b) and no expression in the males (h, k), consistent with RNA-seq. In females it is expressed in the cortex of the ovary, with a lower level in the medulla (e). FGFR3 is also higher in females (c) than males, which show low expression (i – gonads delineated by lines). FGFR3 is mostly expressed in the ovarian cortex (f), and in the male weakly in a subset of cord cells (l, arrow heads). These results are consistent with RNA-seq data. Typically, 3 UGS from each sex were used for each probe, and these images are representative of what is seen. A sense control probe is also tested, and did not show any staining for any of the genes (data not shown)

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