Table 2 Percentage amino acid sequence similarity of mce operons in 43525 compared to M. paratuberculosis K10 (MAP K10), M. avium 104, M. avium TH135, M. intracellulare and M. tuberculosis
MAP K10 | Mav 104 | Mav TH135 | Mav ATCC25291 | M. intracellulare | M. tuberculosis | Remarks | |
|---|---|---|---|---|---|---|---|
Operon | |||||||
mce1 | 99–100 | 77–100 | 99–100 | 81–100 | 92–99 | 76–93 | 3 genes in 43525 are longer, fadD5 (453aa longer than MAP3601), yrbE1B (40aa longer than MAP3603), mce1E (233aa longer than MAP3608 and MavATCC25291_4409 |
mce2 | 99–100 | 97–99 | 99–100 | 97–100a | 62–78 | 72–91 | Mce2E missing in M. avium ATCC25291 |
mce3 | 99–100a | 60–100 | 99 | 98–99 | 84–96 | 50–62 | yrbE3B missing in MAPK10 |
mce4 | 100 | 99–100a | 99–100 | 99–100 | 93–99 | 81–95 | Frameshift in M. avium 104 Mce4F |
mce5 | 99–100a | 99–100a | 65–99a | 90–99 | 85–99 | – | Deletion at position 7862892 of MAPK10 results in truncation of MAP0762 and M. avium ATCC25291_0785 proteins by 241aa and 70aa respectively. Frameshift in Mav0951. |
mce6 | 99–100 | – | – | – | 88–92 | – | Another human MAP strain, MAP4 had 100 % a.a. homology to all genes of 43525 in this operon. |
mce7 | 99–100 | 98–100 | 98–99 | 98–100 | 88–97 | – | This operon also found in M. marinum (>69 % homology) and M. abscessus. |
mce8 | 97–100 | 96–100 | 96–100 | 95–99 | 80–94 | – | Frameshift in MAP K10 (MAP0116). Truncated protein in MavATCC25291_0099. 43525 is 100 % identical to sheep MAP strains S397 and S5 for all genes in this operon. |