Table 1 Results of small RNA sequencing. Counts of: total reads from 18–40 nt; reads mapping to the P. striiformis draft genome; reads remaining after uninfected library subtraction; and reads remaining after removing reads matching wheat miRNA and protein-coding genes
From: Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici)
Treatment | IL | IP | UL | UP | Total |
|---|---|---|---|---|---|
1a. | Â | Â | Â | Â | Â |
Total reads | 12,406,438 | 10,462,148 | 16,947,323 | 16,581,382 | 56,397,291 |
Reads mapping to Pst genome | 190,677 | 176,960 | 196,183 | 185,452 | 749,272 |
Reads mapping to Pst genome (%) | 1.5 | 1.7 | 1.2 | 1.1 | Â |
After subtracting uninfected | 50,909 | 38,362 | -- | -- | 89,271 |
After filtering | 45,754 | 33,805 | -- | -- | 79,559 |
1b. | Â | Â | Â | Â | Â |
Non-redundant sequences | 3,539,878 | 2,921,622 | 4,982,417 | 4,534,040 | 15,977,957 |
Sequences mapping to Pst genome | 44,668 | 39,717 | 29,913 | 30,655 | 144,953 |
Sequences mapping to Pst genome (%) | 1.3 | 1.4 | 0.6 | 0.7 | Â |
After subtracting uninfected | 24,107 | 18,843 | -- | -- | 42,950 |
After filtering | 20,269 | 15,490 | -- | -- | 35,759 |