Table 5 Primer sequences used in RT-PCR experiments
From: Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici)
Primer name | Sequence (5′ to 3′) | Expected product size |
|---|---|---|
PSTG_06326 F | CACTCAGTGTTTTGCCGTGG | 800 |
PSTG_ 06326 R | AGTATGCCGGTGTAGCGATG | |
PSTG_15713 F | GAAGCGTGCAGAGTATTGCG | 887 |
PSTG_15713 R | CAAAGTGTGCGCGGGATATG | |
PSTG_15184 F | GGGTCGAAATCCGCGATAGT | 294 |
PSTG_15184 R | TGGCCGTCCGGTTTAGAATC | |
PSTG_03098 F | TAGCACCAACTGATGCCAGG | 273 |
PSTG_03098 R | AACCTCTTCCAGATGCAGCC | |
PST_actin F | CTATTGACTGAAGCTCCTCTCAATCCCA | 213 |
PST_actin R | CGCATGAGGTAGAGCGTAACCTT | |
TAE_GAPDH F | CAACGCTAGCTGCACCACTA | 161 |
TAE_GAPDH R | TTCCACCTCTCCAGTCCTTG | |
IP_2101 F | TTCCCAGGAAGCGATGAGC | 120 |
IP_2102 F | AGCAGCAACCCTGTCGGC | 120 |
IP_2103 F | TTGCAAGGACTCCGGAGAGGC | 120 |
IP_2105 F | TTTAGCGAGTTGGAGTGAT | 120 |
IP_2106 F | TAGGGACCTCGATAGGACA | 120 |
ta-miR_2002/2075Â F | TGAGATGAGATTACCCCAT | 120 |
U6 snRNA F | CCGATAAAATTGGAACGATAC | 195 |
RTQ-universal R | CGAATTCTAGAGCTCGAGGCAGG | — |
miRTQ | CGAATTCTAGAGCTCGAGGCAGGCGACATGGCTGGCTAGTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCTTTTTTTTTTTTTTTTTTTTTTTTVN | — |