Fig. 6
From: cis-regulatory analysis of the Drosophila pdm locus reveals a diversity of neural enhancers
Sequential activation of the nub-46 enhancer and Castor expression during embryonic NB lineage development. a–d Shown are the dorsal views of whole-mount embryos immunostained for the presence of nub-46 enhancer activity (GFP, green) and Castor (red) expression during NB lineage development. a During mid stage 10 (M10), Castor is present in midline precursors in the ventral nerve cord (VNC), whereas the nub-46 enhancer regulates expression in (a’) a subset of Cas− VNC NBs (arrows). The identification of the VNC NBs are based on size and location. a” There is no co-location of nub-46 enhancer activity and Castor expression. b–b” During early stage 11 (E11), nub-46 enhancer activity and Castor expression begins to co-localize in a subset of VNC NBs (arrowheads). c-c” By mid stage 11 (M11), additional VNC NBs express both Cas and the reporter gene (GFP) driven by nub-46. It is worth noting that co-localization may be in part due to perdurance of GFP. d–d” Co-localization of nub-46 activity and Cas expression is also observed in cephalic lobe cells (arrowheads)