Fig. 7

Enhancer-reporter transgene analysis reveals enhancer regulatory activity during larval CNS and imaginal disc development. Shown are the ventral views of third instar larval CNS whole-mount dissections (a–e) and whole-mount views of third instar imaginal discs (f–h). a–e Similar to nub conserved sequence clusters (CSCs), a subset of pdm-2 CSCs (23 of 59 CSCs) direct mCD8-GFP expression (green) during larval neurogenesis (see Additional file 4: Table S1 for additional larval neural enhancers). a pdm2-17 directs expression in a subset of central brain and lateral VNC neurons. b pdm2-19 activates expression in a collection of neurons in the central brain neurons as well as lateral and medial VNC neurons. c pdm2-35 regulates expression in the medial central brain neurons and posteromedial VNC neurons. d pdm2-39 activates expression in posteromedial VNC neurons. e pdm2-40a directs expression in posterolateral VNC neurons. f–h A subgroup of nub CSCs (5 of 57 CSCs) activates expression in third instar larval imaginal discs including leg, wing, and haltere discs. Shown are the enhancer-reporter transgene expression patterns of nub-31, nub-36, and nub-41