Fig. 8

pdm locus enhancers drives expression in specific classes of larval NBs. a–d pdm enhancers activate reporter expression during larval NB lineage development. Shown is membrane-bound GFP (mCD8-GFP) expression (green) driven by each enhancer. a The nub-46 enhancer regulates central brain and VNC expression. a’ The nub-46 enhancer regulates expression in type I and type II NBs. Shown is a single confocal plane view of a larval brain lobe stained with anti-GFP (green), anti-Ase (red), and anti-Dpn (blue). a” The inset is a magnified view of the yellow dashed square and highlights type I NBs (Ase⁺ Dpn⁺, asterisk), type II NBs (Ase⁻ Dpn⁺, arrowheads), GMCs (Ase⁺ Dpn⁻, arrows) and individual NB lineages (yellow dashed outlines). b The nub-49b enhancer regulates a subset of optic lobe, central brain, and VNC NB lineages. b’ and b” show that nub-49b directs expression in type I and type II NBs. c The pdm2-34 enhancer directs central brain and VNC expression. c’ and c” reveals that pdm2-34 drives expression in type I NBs. d–d” The pdm-37a enhancer activates expression in type I and type II larval NBs