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Table 6 Identification of mRNA targets for miRNAs significant for OS and/or RFS

From: Next generation sequencing profiling identifies miR-574-3p and miR-660-5p as potential novel prognostic markers for breast cancer

miRNA ID Target Identification Gene Ontology
Number of mRNA targets identified from TargetScan Number of predicted targets overlapping with mRNA expression dataset Total number of annotation clusters Number of clusters with enrichment score ≥ 1.3
hsa-miR-15a-5p 1275 181 48 15
hsa-miR-27a-3p 1212 183 47 13
hsa-miR-193b-3p 222 30 4 0
hsa-miR-574-3p 13 2 0 0
hsa-miR-660-5p 149 25 2 1
hsa-miR-210-3p 32 6 1 0
hsa-miR-146b-5p 226 31 4 1
hsa-miR-374a-3p; hsa-miR-374a-5p 680 110 22 9
hsa-miR-221-3p 446 60 25 13
hsa-miR-196a-5p 295 46 13 5
hsa-miR-425-5p 212 24 2 0
  1. Gene targets for 12 miRNAs significant in survival analysis were identified using in silico prediction (TargetScan) and were confirmed with in-house mRNA-miRNA matched expression dataset (n = 17). Gene ontology terms were identified for mRNAs overlapping with in-house dataset using DAVID bioinformatics tool. Only annotation clusters with Enrichment Score (ES) ≥ 1.3 were considered. However, no cluster with ES ≥ 1.3 could be identified for miR-193b-3p, miR-574-3p, miR-210-3p and miR-425-5p