The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

Table 4 Loci encoding well-characterized peptide products, with the highest 5 % πN value between the Kiambu5 stock and the Muguga reference sequence. Variants in hypothetical proteins, and those not in the highest 5 % πN can be found in Additional file 3: Table S3

Locus Tag / 2005	Locus Tag ID^a	Product Name	Domains	Protein Localization/ Confidence^c	πN^d	πN/πS^d	Syn^d	Non-syn^d
TP01_0066	TpMuguga_01g00066	50S ribosomal protein L33	-	S/ 1	0.013	0.4313	3	3
TP01_0248	TpMuguga_01g00248	tRNAHis guanylyltransferase	1 TM^b	_/ 1	0.0148	0.4679	8	10
TP01_0272, TP01_0273	TpMuguga_01g00272	Kinase binding protein CGI-121	-	_/ 1	0.0161	0.3261	7	8
TP01_0374	TpMuguga_01g00374	DEAD/DEAH box helicase	-	_/ 2	0.0131	0.3503	13	15
TP01_0405	TpMuguga_01g00405	Asparagine synthetase domain-containing protein C4F6.11c	-	_/ 1	0.0121	0.4375	14	15
TP01_1056	TpMuguga_01g01056	Merozoite Antigen	1 GPI	S/ 2	0.0125	1.1773	3	8
TP01_1165	TpMuguga_01g01165	ATP-dependent RNA helicase SUV3 homolog mitochondrial	-	M/ 5	0.015	0.3049	25	23
TP01_1173, TP01_1174	TpMuguga_01g01174	Ribonuclease P protein subunit p29	-	_/ 3	0.0123	0.255	7	7
TP01_0275, TP01_0273, TP01_0274, TP01_0272	TpMuguga_01g02345	Phosphatidylinositol 4-phosphate 5-kinase 7	-	_/ 4	0.0142			3
TP01_1063	TpMuguga_01g02455	Uncharacterized protein C18H10.09	-	_/ 1	0.0132	0.66	2	4
TP02_0369	TpMuguga_02g00369	Dual specificity protein phosphatase 12	-	_/ 2	0.0142	0.3435	11	11
TP03_0010	TpMuguga_03g00010	Archease protein family (MTH1598/TM1083)	-	_/ 1	0.0143	0.4618	6	8
TP03_0595	TpMuguga_03g00595	Ydr279p protein family (RNase H2 complex component)	-	_/ 2	0.0187	0.5673	5	13
TP03_0601	TpMuguga_03g00601	CobW/HypB/UreG nucleotide-binding domain	-	_/ 3	0.0127	0.4562	9	10
TP03_0270	TpMuguga_03g02310	Protein CyaY	-	_/ 5	0.0117	0.2878	36	35
TP04_0705	TpMuguga_04g00705	Maf-like protein	-	_/ 3	0.0115	0.5954	5	6

The previously identified antigen p32 is marked in bold
^aLocus Tag Identifiers according to the updated whole genome gene structural re-annotation
^bNumber of transmembrane domains
^cProtein localization predictions based on TargetP: C = chloroplast; M = mitochondrion; S = Secretory pathway; _ = Any other location. Prediction confidence: 1 = max; 5 = min
^dSNPs between Kiambu5 and the reference Muguga isolate

ISSN: 1471-2164