Fig. 2
From: Tools and pipelines for BioNano data: molecule assembly pipeline and FASTA super scaffolding tool
Assembly workflow for assemble_SGE_cluster.pl. a The Irys instrument produces tiff files that are converted into BNX text files. b One BNX file is produced for each flowcell on a IrysChip. c BNX files are split by scan and aligned to the sequence reference. Stretch (bases per pixel) is recalculated for each scan from the alignment. d Quality check graphs are created for each pre-adjusted flowcell BNX. e Adjusted flowcell BNXs are merged. f The first assemblies are run with a variety of p-value thresholds. g The best first assembly (red oval) is chosen and a version of this assembly is produced with a variety of minimum molecule length filters