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Fig. 6 | BMC Genomics

Fig. 6

From: Subtelomere organization in the genome of the microsporidian Encephalitozoon cuniculi: patterns of repeated sequences and physicochemical signatures

Fig. 6

Recombination sites are located close to DNA melting resistant regions. a The state of the DNA molecule between “closed” (double stranded) and “open” (single stranded) was measured at different temperature along the VIIIβ extremity. The GeneFizz software was implementing an algorithm which was able to calculate melting state of the DNA molecule locally [37]. This algorithm had no mathematical horizon (a sliding window of 20 bp was used in the present study). Curves calculated at different temperature were superimposed to identify regions where the transition between closed and open state requires a temperature increase of several degrees (*). Doubled arrows at the top of schema indicate the distance between the recombination site and transition zone. Recombination sites have been mapped in centromere proximal part of a closed region. We never found recombination sites within a closed region, but the distance to a closed region vary from a hundred of base pair to one kbp. The hatched area associated to R03 recombination site covers the region where it is expected to be located. R01 and R02 have been precisely mapped. b Recombination sites are associated with DNA melting resistant regions. The transition between closed and open state of the DNA molecule was plotted in function of the position of the recombination site for all sites at all extremities. Coordinates were calculated from the corresponding end of the consensus sequence

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