Fig. 2

Flow diagram of assigning contigs or singletons to specific circadian clock genes. The functional circadian clock gene was identified in Drosophila melanogaster through Flybase. The Drosophila melanogaster protein sequence was blasted against OrthoDB7 using the most recent common ancestor of all seven species as the search node. The orthologous genes were then taken from the resulting OrthoDB group, with the ortholog of A. aegypti, W. smithii’s most closely related species, and used in a local BLAST against the contigs and singletons from the W. smithii transcriptome. If the lowest E-value from that BLAST identified a single contig or singleton, that contig or singleton was assigned to the respective D. melanogaster gene function in the OrthoDB group. If the lowest E-value from the BLAST identified a multi-gene family, maximum likelihood trees were used to identify the orthologs of various genes in that family (Figs. 3 and 4)