Fig. 8

Effects of DDVP on liver gene expression are distinct from the effects of nutrient restriction. The batch effect due to different experimental dates for DDVP exposure and nutrient deprivation was removed using the batch effect removal tool in Partek Genomics Suite before plotting and clustering. a Principal components analysis (PCA) of 5481 genes differentially expressed in the livers of nutrient restricted or DDVP-exposed fish in comparison with DDVP exposure experiment controls (see text). Nutrient deprived fish form a tight cluster with controls from the DDVP exposure experiment that is well separated from DDVP-exposed fish. The percentage of variance explained by the PCA is shown in parenthesis. b Heat map of the expression levels of the 5481 differentially expressed genes in DDVP-exposed and nutrient deprived fish. Rows are individual genes and columns are pools of 4–5 animals. Expression levels for each gene were standardized to a mean of zero and a standard deviation of one