Fig. 3
GmbZIP38, GmbZI37 and GmbZIP68 activate a BiP promoter. The leaves of transgenic tobacco lines transformed either with a soyBiPD promoter fused to GUS (pBiP-9::GUS) or an empty pCambia vector (pCambia) were agroinfiltrated with plasmids carrying truncated GmbZIP38 (bZIP381–434), GmbZIP37 (bZIP371–406) and GmbZIP68 (bZIP681–209). a Expression of truncated bZIPs in agroinfiltrated leaves. The expression levels of truncated GmbZIP38, GmbZIP37 and GmbZIP68 were analyzed by qRT-PCR at 72 h post-infiltration. Expression levels were calculated using the 2−ΔCT method, with helicase as an endogenous control. The error bars indicate 95 % confidence intervals based on t-tests (p < 0.05, n = 3). b Induction of GUS activity in transgenic lines by expression of truncated GmbZIP38, GmbZIP37 and GmbZIP68. Transgenic tobacco leaves (pBiP-9::GUS and pCambia) were infiltrated with A. tumefaciens carrying the indicated DNA constructs, and GUS activity was measured at 72 h post-infiltration. Non-inoculated (SI) transgenic lines and those inoculated with either GV3101 or GFP were used as negative controls. c GUS transcript accumulation. The expression of GUS was analyzed by qRT-PCR at 72 h post-infiltration. Expression levels were calculated using the 2−ΔCT method, and helicase served as an endogenous control. The error bars indicate 95 % confidence intervals based on t-tests (p < 0.05, n = 3)