Fig. 2

Strand-specific PCR of 17 randomly selected lncRNAs to determine the transcription orientation. BPHOGS10035448-AS-RA was not amplified with a correct band. So, 16 lncRNAs were successfully amplified and confirmed by sequencing (see Figure S2 for RT-PCR validation). The results indicated that 12 lncRNA transcribed from the antisense strand, and four from the sense strand. F: forward primer (sense); R: reverse primer (antisense); RT: reverse transcriptase