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Table 1 Strains and plasmids used for clonal, genomic, and phenotypic analyses during this study

From: Fatty acid kinase A is an important determinant of biofilm formation in Staphylococcus aureus USA300

Strains/Plasmids Description Source
Strains
UA S391 S. aureus strain USA_UAS391 [18]
JE2 S. aureus USA300 parental strain for the NARSA transpos on library NARSAa
FPR3757 S. aureus USA300_FPR3757 NARSA
TCH1516 S. aureus subsp. aureus USA300_TCH1516 ATCC
UA S391-Erys Heat-cured erythromycin-sensitive derive of UAS391 This study
TCH1516-Erys Heat-cured erythromycin-sensitive derive of TCH1516 This study
UA S391-NE229 UA S391-Erys transductant with mutation in EX97_05885 This study
TCH1516-NE229 TCH1516-Erys transductant with mutation in USA 300Hou_1162 This study
NE229-pHD954 S. aureus stra NE229 completed with pHD954 This study
NE229-pHD957 S. aureus strain NE229 completed with pHD957 This study
UA S391-pHD957 S. aureus strain UA S391 completed with pHD957 This study
RN0451 Phage Φ11 lysogenic S. aureus strain (NARSA strain NRS 136) NARSA
RN0450 S. aureus RN0450 (NARSA strain NRS 135) NARSA
RN4220 Restriction-deficient intermediate S. aureus cloning host RN4420 NARSA
  Restriction-deficient intermediate S. aureus cloning host RN4420 NARSA
NE1646 Transposon mutant with insertion in USA200HOU_0155 (SA USA300_0145) NARSA
NE229 Transpos on mutant with insertion in USA300HOU_1162 (SA USA300_1119) NARSA
NE1081 Transpo on mutant with insertion in USA300HOU_2626 (SA USA300_2561) NARSA
NE454 Transpo on mutant with insertion in USA300HOU_2631 (SA USA300_2566) NARSA
NE1290 Transposon mutant with insertion USA300HOU_2641 (SA USA300_2576) NARSA
NE81 Transposon mutant with insertion in USA300HOU_2319 (SA USA300_2285) NARSA
NE1036 Transposon mutant with insertion in USA300HOU_2602 (SA USA300_2542) NARSA
NE1038 Transposon mutant with insertion in USA300HOU_2678 (SA USA300_2610) NARSA
NE33 Transposon mutant with insertion in USA300HOU_2654 (SA USA300_2589) NARSA
NE1262 Transposon mutant with insertion in USA300HOU_2026 (SA USA300_1984) NARSA
NE1875 Transposon mutant with insertion in USA300HOU_1934 (SA USA300_1918) NARSA
NE1 Transposon mutant with insertion in USA300HOU_1372 (SaA USA300_1327) NARSA
NE334 Transposon mutant with insertion in USA300HOU_2197 (SA USA300_2161) NARSA
NE809 Transposon mutant with insertion in USA300HOU_1260 (SA USA300_1214) NARSA
NE1403 Transposon mutant with insertion in USA300HOU_1260 (SA USA300_1585) NARSA
NE1026 Transposon mutant with insertion in USA 300HOU_1338 (SA USA 300_1298) NARSA
NE1314 Transposon mutant with insertion in USA300HOU_0953(SA USA300_0896) NARSA
Plasmids
pALC2073 Shuttle vector pALC2073 [11]
pHD954 pALC2073 with cloned fakA gene amplifie from UAS391 This study
pHD957 pALC2073 with cloned fakA gene amplifie from TCH15116 This study
  1. aThis library now exists at www.beiresources.org and is discussed by Fey et al. [8]