Table 1 Strains and plasmids used for clonal, genomic, and phenotypic analyses during this study
From: Fatty acid kinase A is an important determinant of biofilm formation in Staphylococcus aureus USA300
Strains/Plasmids | Description | Source |
|---|---|---|
Strains | ||
UA S391 | S. aureus strain USA_UAS391 | [18] |
JE2 | S. aureus USA300 parental strain for the NARSA transpos on library | NARSAa |
FPR3757 | S. aureus USA300_FPR3757 | NARSA |
TCH1516 | S. aureus subsp. aureus USA300_TCH1516 | ATCC |
UA S391-Erys | Heat-cured erythromycin-sensitive derive of UAS391 | This study |
TCH1516-Erys | Heat-cured erythromycin-sensitive derive of TCH1516 | This study |
UA S391-NE229 | UA S391-Erys transductant with mutation in EX97_05885 | This study |
TCH1516-NE229 | TCH1516-Erys transductant with mutation in USA 300Hou_1162 | This study |
NE229-pHD954 | S. aureus stra NE229 completed with pHD954 | This study |
NE229-pHD957 | S. aureus strain NE229 completed with pHD957 | This study |
UA S391-pHD957 | S. aureus strain UA S391 completed with pHD957 | This study |
RN0451 | Phage Φ11 lysogenic S. aureus strain (NARSA strain NRS 136) | NARSA |
RN0450 | S. aureus RN0450 (NARSA strain NRS 135) | NARSA |
RN4220 | Restriction-deficient intermediate S. aureus cloning host RN4420 | NARSA |
| Â | Restriction-deficient intermediate S. aureus cloning host RN4420 | NARSA |
NE1646 | Transposon mutant with insertion in USA200HOU_0155 (SA USA300_0145) | NARSA |
NE229 | Transpos on mutant with insertion in USA300HOU_1162 (SA USA300_1119) | NARSA |
NE1081 | Transpo on mutant with insertion in USA300HOU_2626 (SA USA300_2561) | NARSA |
NE454 | Transpo on mutant with insertion in USA300HOU_2631 (SA USA300_2566) | NARSA |
NE1290 | Transposon mutant with insertion USA300HOU_2641 (SA USA300_2576) | NARSA |
NE81 | Transposon mutant with insertion in USA300HOU_2319 (SA USA300_2285) | NARSA |
NE1036 | Transposon mutant with insertion in USA300HOU_2602 (SA USA300_2542) | NARSA |
NE1038 | Transposon mutant with insertion in USA300HOU_2678 (SA USA300_2610) | NARSA |
NE33 | Transposon mutant with insertion in USA300HOU_2654 (SA USA300_2589) | NARSA |
NE1262 | Transposon mutant with insertion in USA300HOU_2026 (SA USA300_1984) | NARSA |
NE1875 | Transposon mutant with insertion in USA300HOU_1934 (SA USA300_1918) | NARSA |
NE1 | Transposon mutant with insertion in USA300HOU_1372 (SaA USA300_1327) | NARSA |
NE334 | Transposon mutant with insertion in USA300HOU_2197 (SA USA300_2161) | NARSA |
NE809 | Transposon mutant with insertion in USA300HOU_1260 (SA USA300_1214) | NARSA |
NE1403 | Transposon mutant with insertion in USA300HOU_1260 (SA USA300_1585) | NARSA |
NE1026 | Transposon mutant with insertion in USA 300HOU_1338 (SA USA 300_1298) | NARSA |
NE1314 | Transposon mutant with insertion in USA300HOU_0953(SA USA300_0896) | NARSA |
Plasmids | ||
pALC2073 | Shuttle vector pALC2073 | [11] |
pHD954 | pALC2073 with cloned fakA gene amplifie from UAS391 | This study |
pHD957 | pALC2073 with cloned fakA gene amplifie from TCH15116 | This study |