Fig. 2

Next-Generation Sequencing (NGS) of messenger RNA from Uterine Biopsies at both 7 and 21 DPP. a top panel High numbers of genes are differentially expressed in the endometrium from the HC (healthy) group between 7 and 21 DPP, whereas (bottom panel) the same transition in gene expression profile between time points is not present in cows that develop CE. The numbers of significantly differentially expressed genes between groups at both postpartum time points are shown (FDR < 0.1). Within group comparisons are shown in red boxes, and between group comparisons are in blue boxes. The numbers in green ovals show the direction in relative gene expression between comparisons. b MDS plots generated from endometrial RNA-seq data shows clearer clustering of 7 and 21 DPP time points for the HC group (top panel) than for the CE group (bottom panel). Nine HC (healthy control) endometrial samples at both 7 DPP (red) and corresponding same animal sample at 21 DPP (green) and b six CE (cytologically endometritic) cows at the same two time points are shown. Corresponding gene lists are shown in Additional file 2: Table S2