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Table 5 R-M systems of B. longum subsp. longum NCIMB 8809 and B. longum subsp. longum CCUG 30698

From: Pangenome analysis of Bifidobacterium longum and site-directed mutagenesis through by-pass of restriction-modification systems

Strain

R-M type

Locus tag

Predicted function

REBASE comparison

Predicted associated motif

nSites on pAM5

B. longum subsp. longum NCIMB 8809

I

B8809_1350 (S3.blmNCI)

HsdS subunit

100 % with Bbr12LORF1341

5'–GTNNNNNTGCC–3'/

1

B8809_1351

Recombinase

-

B8809_1352(S2.blmNCI)

HsdS subunit

100 % with Bbr12LORF1345

3'CTNNNNNACGG–5'*

B8809_1353(S1.blmNCI)

HsdS subunit

100 % with Bbr12LORF1345

B8809_1354(M.blmNCI)

MTase

100 % with BloF8ORF1960P

B8809_1355 (R.blmNCI)

RTase

100 % with BloF8ORF1960P

II

B8809_0606 (R.blmNCII)

RTase

90 % with Blo68ORF557P

5' – CWGG – 3'

13

B8809_0607(M.blmNCII)

MTase

82 % with Blo68ORF556P, N5-C

II

B8809_0959 (M.blmNCIII)

MTase

67 % with Bde27679ORF2230P, N4-C

5' – TCGGCGA – 3'*

0

B. longum subsp. longum CCUG 30698

I

BBL306_1749

HsdS subunit

42 % with Cty7086ORFEP

5' –ACCNNNNNRTTG– 3'/ 3' –TGGNNNNNYAAC– 5'*

2

BBL306_1750

Recombinase

-

BBL306_1751

HsdS subunit

44 % with BkaPV202ORF3675

BBL306_1752

Hypothetical protein

-

BBL306_1753

MTase

98 % with Blo44BORF2305P

5'–GCNNNNNNNNTGC–3'*

4

BBL306_1754

HsdS subunit

67 % with BloDORF1956P

BBL306_1756

RTase

100 % with BloDORF1959P

II

BBL306_0230

RTase

100 % with BloAORF289P

5' – CTGCG – 3'*

1

BBL306_0231

MTase

68 % with BbrUIII, N6-A

II

BBL306_0271

MTase

100 % with M.Blo7210ORFAP, N6-A

Unknown

Unknown

II

BBL306_1693

MTase

10 % Pae9BRORFBP,N4-C/N6-A

Unknown

Unknown

IIG

BBL306_1028

RTase/MTase

98 % with Blo1217ORF992P, N6-A

5' – CGGGG – 3'*

5

III

BBL306_0910

MTase

45 % with Bwa914ORF4853, N6-A

Unknown

Unknown

  1. Methylated bases are indicated in bold red text. The “*” indicates sites that were identified by methylome analysis