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Table 5 R-M systems of B. longum subsp. longum NCIMB 8809 and B. longum subsp. longum CCUG 30698

From: Pangenome analysis of Bifidobacterium longum and site-directed mutagenesis through by-pass of restriction-modification systems

Strain R-M type Locus tag Predicted function REBASE comparison Predicted associated motif nSites on pAM5
B. longum subsp. longum NCIMB 8809 I B8809_1350 (S3.blmNCI) HsdS subunit 100 % with Bbr12LORF1341 5'–GTNNNNNTGCC–3'/ 1
B8809_1351 Recombinase -
B8809_1352(S2.blmNCI) HsdS subunit 100 % with Bbr12LORF1345
3'CTNNNNNACGG–5'*
B8809_1353(S1.blmNCI) HsdS subunit 100 % with Bbr12LORF1345
B8809_1354(M.blmNCI) MTase 100 % with BloF8ORF1960P
B8809_1355 (R.blmNCI) RTase 100 % with BloF8ORF1960P
II B8809_0606 (R.blmNCII) RTase 90 % with Blo68ORF557P 5' – CWGG – 3' 13
B8809_0607(M.blmNCII) MTase 82 % with Blo68ORF556P, N5-C
II B8809_0959 (M.blmNCIII) MTase 67 % with Bde27679ORF2230P, N4-C 5' – TCGGCGA – 3'* 0
B. longum subsp. longum CCUG 30698 I BBL306_1749 HsdS subunit 42 % with Cty7086ORFEP 5' –ACCNNNNNRTTG– 3'/ 3' –TGGNNNNNYAAC– 5'* 2
BBL306_1750 Recombinase -
BBL306_1751 HsdS subunit 44 % with BkaPV202ORF3675
BBL306_1752 Hypothetical protein -
BBL306_1753 MTase 98 % with Blo44BORF2305P 5'–GCNNNNNNNNTGC–3'* 4
BBL306_1754 HsdS subunit 67 % with BloDORF1956P
BBL306_1756 RTase 100 % with BloDORF1959P
II BBL306_0230 RTase 100 % with BloAORF289P 5' – CTGCG – 3'* 1
BBL306_0231 MTase 68 % with BbrUIII, N6-A
II BBL306_0271 MTase 100 % with M.Blo7210ORFAP, N6-A Unknown Unknown
II BBL306_1693 MTase 10 % Pae9BRORFBP,N4-C/N6-A Unknown Unknown
IIG BBL306_1028 RTase/MTase 98 % with Blo1217ORF992P, N6-A 5' – CGGGG – 3'* 5
III BBL306_0910 MTase 45 % with Bwa914ORF4853, N6-A Unknown Unknown
  1. Methylated bases are indicated in bold red text. The “*” indicates sites that were identified by methylome analysis
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BMC Genomics

ISSN: 1471-2164

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