Gene duplication prediction by clustering of Acropora digitifera predicted toxins. Clustering was performed using the HHCompare tool and verified by Maximum Likelihood and Maximum Parsimony based methods. Groups marked with * are detected by Maximum Likelihood and Maximum Parsimony based clustering, while groups marked ! were not detected. Proteins highlighted in yellow were detected by high throughput mass spectrometric protein analysis of soluble proteins from discharged nematocysts.