Fig. 3
Representative images of phenotypes observed following RNAi knockdown. a RNAi feeding schedules. Phenotypes in panels (b), (c), and (g) appeared after three feedings, panel (l) after 12 feedings, and the remainder after six feedings. b-c Loss of neoblasts in Smed-slbp(RNAi). Arrow indicates reduced-size blastema. Anti-PH3 labels the mitotic neoblasts. Live animals were imaged on day seven of regeneration, and stained animals were fixed on day 14. d-e Phenotypes of Smed-dkc1(RNAi). Arrows indicate missing blastemas. d = day seven following pre-pharyngeal amputation, e = ventral curling and lysis on day 14. f Impaired regeneration following six Smed-rbap46/48-2 dsRNA feedings. Animals imaged on day seven. g Abnormally elongated photoreceptor pigment (yellow triangles) in day seven regenerates following three feedings against Smed-rbap46/48-2. h Asymmetric photoreceptors of Smed-mcm2(RNAi). The red triangle indicates underdeveloped photoreceptor in day six regenerates. i Reduced blastema (arrow) and underdeveloped photoreceptors (red triangles) in day six Smed-fen1(RNAi) regenerates. j Reduced blastema (arrow) and forked tail (blue triangle) in day seven Smed-ptbp1(RNAi) regenerates. k Smed-tph(RNAi) animals lacking photoreceptor pigment on day 14 of regeneration. Red triangles indicate missing pigment. l Reduction of photoreceptor pigment in Smed-ddc(RNAi). Red triangles indicate photoreceptor pigment cups. m Elongated body shape following extended Smed-tph RNAi. n Edema following Smed-gas8 RNAi. The animal shown is affected in the posterior. o-q Smed-pgbd4(RNAi) phenotypes. White arrows indicate dorsal humps in uninjured animals (o) and day 14 regenerates (p). The blue triangle indicates a post-pharyngeal lesion, and the green arrowhead indicates lateral bulging. q WISH to pharynx marker laminin in Smed-pgbd4(RNAi). Upper animals are shown ventrally, and lower animals were imaged from the side, with dorsal toward the top. Anterior is to the left. Yellow arrows and arrowheads indicate the base of the pharynx and its ventral opening, respectively. Brackets indicate the pharynx in controls. Scale = 0.5 mm for all panels