Fig. 2

Robustness and reproducibility of transcriptomic analysis for T. hominis. In this figure, each sample is an individual biological replicate of T. hominis infected RK-13 cells from which total RNA was purified. All of these samples were sequenced on two different lanes of the Illumina sequencing chip to provide technical replication. The strong positive correlation of transcript abundance, as measured by Fragments Per Kilobase per Million mapped reads (FPKM) between replicates in all cases is indicative of high levels of reproducibility between both biological and technical replicates. a Pairwise comparisons of T. hominis transcript log₁₀ FPKM values between biological replicates (samples) and technical replicates (sequencing lanes); density plots generated in cummeRbund [109] represent the distribution of log₁₀ FPKM values for that replicate. b FPKM density plot overlay comparing the distribution of T. hominis transcript FPKM values between individual biological replicates (samples) from a single sequencing lane. c FPKM density plot overlay comparing the distribution of T. hominis transcript FPKM values between technical replicates (different sequencing lanes) from a single sample (Sample 1). The levels of reproducibility between replicates for the expression of host genes were equally very high, this data is summarised in Additional file 1, Figure S1