Fig. 9

Analysis of transactivation activities and subcellular localization of SlbZIP6, SlbZIP12, SlbZIP16, SlbZIP32 and SlbZIP46. a Transactivation activity of SlbZIP6, SlbZIP12, SlbZIP16, SlbZIP32 and SlbZIP46 in yeast. Yeasts carrying pBD-SlbZIP6/12/16/32/46 or pBD empty vector (as a negative control) were streaked on the SD/Trp⁻ plates (left) or SD/Trp⁻ His⁻ plates (middle) or SD/Trp⁻His⁻/X-α-Gal plates (right) for 3 days at 30 °C. b Subcellular localization of SlbZIP6, SlbZIP12, SlbZIP16, SlbZIP32 and SlbZIP46. Agrobacteria carrying SlbZIP6, SlbZIP12, SlbZIP16, SlbZIP32, SlbZIP46 or control vector GFP were infiltrated into leaves of N. benthamiana plants and the fluorescence images were taken in dark field for green fluorescence (left), in dark field for red fluorescence (nuclear maker) (middle left), in white field for the morphology of the cell (middle right), and in combination (right), respectively. Scale bar = 20 μm