Fig. 6
From: Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315
Expression of the kdp-system in B. cenocepacia J2315 biofilms. Panel a: dRNA-Seq and gRNA-Seq data for the kdp-operon. dRNA-Seq data are represented as read starts per base, gRNA-Seq data are represented as coverage. Red arrows: pTSS, at position 2640727 (kdpA) and 2635837 (kdpD). Panel b: Quantitative RT-PCR of kdpA in biofilms and planktonic cultures. Bars represent expression relative to planktonic logarithmic cultures and are based on three biological replicates. Orange: Planktonic culture in logarithmic phase, harvested at 5 × 108 CFU/ml. Light blue: Planktonic culture in late logarithmic phase, harvested at 1 × 109 CFU/ml. Brown: Planktonic culture in stationary phase, harvested at 2.2 × 109 CFU/ml. Green: Biofilm grown in microtiter plates