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Table 4 Differential expression analysis for 454 data: transcripts expressed ≥ 10-fold more highly in glowworm light organ tissue than in non-light organ tissue

From: Comparative RNA seq analysis of the New Zealand glowworm Arachnocampa luminosa reveals bioluminescence-related genes

  

Light organ

Rest of body

   

Contig number

Gene length (bp)

Total gene reads

Normalized expression values

RPKM

Total gene reads

Normalized expression values

RPKM

Difference (normalized values)

Fold change (normalized values)

Putative identity from BLASTX search hits

16656

711

955

2387.2

2876.3

2

7.4

5.9

2379.8

322.6

phosphatidylethanolamine-binding protein

12303

1832

1702

1651.2

1989.5

9

13

10.3

1638.2

127.0

luciferin 4-monooxygenase

10195

478

2588

9622.5

11594.2

14

77.4

61.6

9545.1

124.3

luciferin 4-monooxygenase

13441

1452

13014

15929.3

19193.3

87

158.4

126.0

15770.9

100.6

luciferin 4-monooxygenase

12283

1279

446

619.8

746.7

3

6.2

4.9

613.6

100.0

glutathione s-transferase

4637

1047

273

463.4

558.4

2

5.1

4.0

458.3

90.9

sulfotransferase

12325

1830

2569

2495

3006.2

21

30.3

24.1

2464.7

82.3

luciferin 4-monooxygenase

2377

1303

339

462.4

557.1

4

8.1

6.5

454.3

57.1

aminoacylase-1

2684

2024

65

57.1

68.8

1

1.3

1.0

55.8

43.9

otopetrin

17552

746

513

1222.2

1472.6

8

28.4

22.5

1193.8

43.0

cleavage stimulation factor 64 kDa subunit

11885

2198

49

39.6

47.7

1

1.2

1.0

38.4

33.0

ATP-binding cassette transporter

3008

1206

49

72.2

87.0

1

2.2

1.7

70

32.8

GST-containing flywch zinc-finger protein

16267

1647

42

45.3

54.6

1

1.6

1.3

43.7

28.3

protein lsm14 homolog b-like

12345

961

293

541.9

652.9

7

19.3

15.3

522.6

28.1

carboxylesterase

2668

928

72

137.9

166.1

2

5.7

4.5

132.2

24.2

tpa_inf: hdc07468

12310

1030

452

779.9

939.7

13

33.4

26.5

746.5

23.4

carboxylesterase

4414

2035

138

120.5

145.2

4

5.2

4.1

115.3

23.2

sodium-dependent phosphate transporter

4376

704

32

80.8

97.3

1

3.8

3.0

77

21.3

carbonic anhydrase

11948

1363

29

37.8

45.6

1

1.9

1.5

35.9

19.9

facr2_drome ame: full = fatty acyl- reductase cg8303

16727

1498

30

35.6

42.9

1

1.8

1.4

33.8

19.8

short-chain dehydrogenase

16747

1127

28

44.2

53.2

1

2.3

1.9

41.9

19.2

cofilin actin-depolymerizing factor homolog

3316

1969

21

19

22.8

1

1.3

1.1

17.7

14.6

isoform a

9802

1035

22

37.8

45.5

1

2.6

2.0

35.2

14.5

kynurenine aminotransferase

9665

1782

19

18.9

22.8

1

1.5

1.2

17.4

12.6

transposable element tc3 transposase

11695

1953

56

51

61.4

3

4.1

3.2

46.9

12.4

protein yellow

12223

1142

36

56

67.5

2

4.6

3.7

51.4

12.2

ganglioside-induced differentiation-associated protein 1

10010

267

18

119.8

144.4

1

9.9

7.9

109.9

12.1

hypothetical protein Sulku_2095

3263

1410

17

21.4

25.8

1

1.9

1.5

19.5

11.3

multiple inositol polyphosphate phosphatase

4985

851

16

33.4

40.3

1

3.1

2.5

30.3

10.8

ubiquitin fusion degradaton protein

13509

589

32

96.6

116.3

2

9

7.1

87.6

10.7

kda midgut protein

16372

1493

16

19

22.9

1

1.8

1.4

17.2

10.6

cytochrome p450

4731

700

31

78.7

94.8

2

7.6

6.0

71.1

10.4

isoform b

7053

817

15

32.6

39.3

1

3.2

2.6

29.4

10.2

No significant similarity found

16679

960

45

83.3

100.4

3

8.3

6.6

75

10.0

hypothetical conserved protein

  1. Transcripts are ranked according to normalized expression values; read counts were scaled so that the median values were made equal. RPKM = reads per kilobase of exon model per million mapped reads