Fig. 2

Genome-wide mapping of candidate genetic loci by measurement of relative heterozygosity in sequenced phenotype pools. Plots depict heterozygosity measures on chromosome 3 L. a Total pooled heterozygosity (Hp) was calculated in a sliding 10 kb window along chromosome 3 L within the Fd03 founder colony. Dots indicate minimum and maximum values for a 1 Mb window, the black line indicates the average heterozygosity and the gray shading represents the standard deviation of total pooled heterozygosity across a 1 Mb window. b Relative diversity (HpR) per 1 Mb window, calculated as the proportion of heterozygosity in a given pool relative to total heterozygosity within the source Fd03 founder colony. Color of point indicates per window elevated heterozygosity (blue lines), or reduced heterozygosity (red lines), plotted as log base 10 of the relative diversity. Phenotype pool identity is indicated in the lower left of each panel (high, low, zero oocysts). A relative heterozygosity value of HpR = 1 indicates the same heterozygosity levels in tested pool as compared to all other pools, values of HpR > 1 indicate greater heterozygosity in the tested pool and values of HpR < 1 indicate lower heterozygosity in the tested pool. On the log scale y-axis, values of 0.5 and 2.0 are equidistant from 1. Candidate locus 3.1 is indicated by the red vertical shaded bar at coordinates 17.4-19.1 Mb. In this interval, relative heterozygosity is increased in the high pool and simultaneously reduced in the low and zero pools