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Table 3 Highly over-expressed genes identified in the white isthmus during deposition of the eggshell membranesa

From: Identifying specific proteins involved in eggshell membrane formation using gene expression analysis and bioinformatics

Gene symbol

Entrez gene name

WI/Ut (fold change)

WI/Ma (fold change)

Secretion

Confirmed by proteomics

SignalPb

SecretomePc

ESd membranes

ESd matrix

COL10A1

Collagen α-1(X) chain-like

111.43; 50.21

155.42; 94.35

Yes

-

[7, 17, 18]

[6, 35, 38]

R3HCC1

R3H domain and coiled-coil containing 1

79.34

136.24

-

-

-

-

CREMP

PREDICTED: similar to spore coat protein SP75, partial

70.03

58.08

-

Yes

[7, 17, 18]

-

AP1G1

Adaptor-related protein complex 1, gamma 1 subunit

45.89

15.56

-

-

-

-

FBN1

Fibrillin 1

45.89

2.53

-

-

-

-

FCGBP

IgGFc-binding protein-like

22.63; 7.62

34.06; 8.82

Yes

-

-

-

MXI1

MAX interactor 1

14.12

9.85

-

-

-

-

LIMCH1

LIM and calponin homology domains-containing protein 1

11.63

15.45

-

-

-

-

FAM164C

Family with sequence similarity 164, member C

3.71

2.79

-

Yes

-

-

TDRD9

putative ATP-dependent RNA helicase

3.36

2.68

-

Yes

-

-

HPX

Hemopexin

2.95

1.57

-

Yes

[7, 17, 18]

[6, 35, 38]

TMEM63C

Transmembrane protein 63C

2.58

2.16

-

-

-

-

SORD

Sorbitol dehydrogenase

2.48

2.25

-

Yes

-

-

PHGDH

D-3-phosphoglycerate dehydrogenase

1.79

2.39

-

Yes

-

-

TXN

Thioredoxin

1.66

2.06

-

-

[7, 17]

[35]

  1. aHigh over-expression defined as ≥ 2-fold (Log2 Ratio ≥ 1.0) relative to either the magnum or the uterus
  2. bPredicted by SignalP 4.1 software
  3. cPredicted by SecretomeP 2.0 software
  4. dES Eggshell