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Fig. 1 | BMC Genomics

Fig. 1

From: A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

Fig. 1

Organization and expression of the Type II-C CRISPR-cas locus of B. pseudohinzii. a Graphical representation of the CRISPR-cas locus. The red block upstream of the cas9 gene is a putative tracrRNA flanked by the predicted promoter (arrow) and stem-loop terminator (up-side-down sigma symbol). The CRISPR array is enlarged relative to the cas genes for visual clarity. b Nucleotide sequence of the CRISPR array. SP# is the spacer sequence number; DR# is the direct repeat number. Nucleotides deviating from the DR consensus in DR-1 and DR-4 are highlighted in red. c Confirmation of cas9 (C9), cas1 (C1), and cas2 (C2) expression during logarithmic (Log) and stationary phases of growth. Each PCR amplicon was designed to have a similar size. M: 100-bp DNA ladder. d The CRISPR array is processed into individual, mature crRNAs. Positive and negative strands are relative to the orientation shown in Fig. 1a. M: 50-bp DNA ladder

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