A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

Table 1 Highest-scoring BLASTn hits for spacer sequences

BLASTn			Sp#	%ID	%cov	%HQ
Protospacer	Locus tag/name	Organism	Sp#	%ID	%cov	%HQ
Prophage: hypothetical protein	L544_3238	Bordetella hinzii OH87 BAL007II	8	100	100	100
Prophage: intergenic region	L544_1114	Bordetella hinzii OH87 BAL007II	9	100	100	100
Prophage: intergenic region	L544_1115	Bordetella hinzii OH87 BAL007II	9	100	100	100
Prophage: replicative DNA helicase	L541_4891	Bordetella hinzii CA90 BAL1384	16	100	97	97
Prophage: replicative DNA helicase	dnaB_2	Bordetella hinzii CA90 BAL1384	16	100	97	97
Mobile element of IS3/IS911 family	Transposase	Variovorax paradoxus EPS	13	90	100	90
Pyruvate carboxyl transferase	C791_4721	Amycolatopsis azurea DSM 43854	6	93	97	90
transfer RNA	tRNA-Gly	Bifidobacterium asteroides PRL2011	1	93	93	86
Prophage: Siphovirus gp157	BB3535	Bordetella bronchiseptica RB50	10	93	93	86
Phage structural component	KF689531.1	Gokushovirinae clone BBHD08n1	20	96	86	83
Phage structural component	major capsid	Gokushovirinae clone BBHD08n1	20	96	86	83

Sp# spacer number, %ID percent nt identity, %cov percent coverage, %HQ percent hit quality

ISSN: 1471-2164