Skip to main content

Table 1 Plant responses (nodule number, n°/plant) and shoot dry weight (g/pl) to inoculation of common bean, leucaena, siratro, Lotus japonicus and L. burtii with R. tropici strain CIAT 899 and derivatives. Plants evaluated after 25 (common bean.), 42 days (leucaena and siratro), or 50 days (Lotus spp.) of growth under controlled conditions

From: Opening the “black box” of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899

Strains P. vulgaris L. leucocephala M. atropurpureum L. japonicus L. burtii
Nodule number Shoot dry weight Nodule number Shoot dry weight Nodule number Shoot dry weight Nodule number Shoot dry weight Nodule number Shoot dry weight
R. tropici CIAT 899 213 ± 52a 1.82 ± 0.64a 13 ± 4a 0.41 ± 0.03a 34 ± 8a 0.05 ± 0.01a 22 ± 9 0.064 ± 0.03 11 ± 5 0.04 ± 0.02
nodD1 mutant 38 ± 11*,a 1.42 ± 0.35a 0 ± 0*,a 0.09 ± 0.01*,a 0 ± 0*,a 0.05 ± 0a 0 ± 0* 0.007 ± 0.004* 5 ± 2* 0.04 ± 0.02
nodD2 mutant 95 ± 38*,a 1.03 ± 0.27a 10 ± 3a 0.36 ± 0.04**,a 24 ± 8a 0.05 ± 0a 30 ± 10 0.039 ± 0.013* 12 ± 5 0.03 ± 0.01
nodD3 mutant 182 ± 25 1.80 ± 0.34 13 ± 3 0.39 ± 0.09 38 ± 12 0.05 ± 0 12 ± 4* 0.025 ± 0.014* 9 ± 4 0.04 ± 0.01
nodD4 mutant 190 ± 24 1.84 ± 0.5 14 ± 3 0.37 ± 0.01* 29 ± 8 0.05 ± 0 17 ± 6 0.058 ± 0.052 12 ± 5 0.03 ± 0.02
nodD5 mutant 179 ± 16 1.45 ± 0.68 12 ± 4 0.37 ± 0.01* 33 ± 7 0.05 ± 0.01 6 ± 2* 0.021 ± 0.011* 12 ± 2 0.05 ± 0.01
None 0 ± 0* 0.80 ± 0.25* 0 ± 0* 0.09 ± 0.01* 0 ± 0* 0.05 ± 0 0 ± 0* 0.008 ± 0.004* 0 ± 0* 0.01 ± 0*
  1. aAfter [23]
  2. *Data represent means ± SD (standard deviation) of 6 jars, each with two plants. nodD3, nodD4 and nodD5 mutant parameters were individually compared with the parental strain CIAT 899 parameters by using the Mann-Whitney non-parametric test. Values tagged by * and ** are significantly different at the level α = 10 and 5 %, respectively