Opening the “black box” of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899

BMC Genomics

Table 1 Plant responses (nodule number, n°/plant) and shoot dry weight (g/pl) to inoculation of common bean, leucaena, siratro, Lotus japonicus and L. burtii with R. tropici strain CIAT 899 and derivatives. Plants evaluated after 25 (common bean.), 42 days (leucaena and siratro), or 50 days (Lotus spp.) of growth under controlled conditions

Strains	P. vulgaris		L. leucocephala		M. atropurpureum		L. japonicus		L. burtii
Strains	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight
R. tropici CIAT 899	213 ± 52^a	1.82 ± 0.64^a	13 ± 4^a	0.41 ± 0.03^a	34 ± 8^a	0.05 ± 0.01^a	22 ± 9	0.064 ± 0.03	11 ± 5	0.04 ± 0.02
nodD1 mutant	38 ± 11^*,a	1.42 ± 0.35^a	0 ± 0^*,a	0.09 ± 0.01^*,a	0 ± 0^*,a	0.05 ± 0^a	0 ± 0^*	0.007 ± 0.004^*	5 ± 2^*	0.04 ± 0.02
nodD2 mutant	95 ± 38^*,a	1.03 ± 0.27^a	10 ± 3^a	0.36 ± 0.04^**,a	24 ± 8^a	0.05 ± 0^a	30 ± 10	0.039 ± 0.013^*	12 ± 5	0.03 ± 0.01
nodD3 mutant	182 ± 25	1.80 ± 0.34	13 ± 3	0.39 ± 0.09	38 ± 12	0.05 ± 0	12 ± 4^*	0.025 ± 0.014^*	9 ± 4	0.04 ± 0.01
nodD4 mutant	190 ± 24	1.84 ± 0.5	14 ± 3	0.37 ± 0.01^*	29 ± 8	0.05 ± 0	17 ± 6	0.058 ± 0.052	12 ± 5	0.03 ± 0.02
nodD5 mutant	179 ± 16	1.45 ± 0.68	12 ± 4	0.37 ± 0.01^*	33 ± 7	0.05 ± 0.01	6 ± 2^*	0.021 ± 0.011^*	12 ± 2	0.05 ± 0.01
None	0 ± 0^*	0.80 ± 0.25^*	0 ± 0^*	0.09 ± 0.01^*	0 ± 0^*	0.05 ± 0	0 ± 0^*	0.008 ± 0.004^*	0 ± 0^*	0.01 ± 0^*

^aAfter [23]
^*Data represent means ± SD (standard deviation) of 6 jars, each with two plants. nodD3, nodD4 and nodD5 mutant parameters were individually compared with the parental strain CIAT 899 parameters by using the Mann-Whitney non-parametric test. Values tagged by ^* and ^** are significantly different at the level α = 10 and 5 %, respectively

ISSN: 1471-2164