Fig. 3

Comparison of P. falciparum K1 DHA-responsive genes from microarray and RNA-seq data. The genes identified as showing significant changes in expression in response to 1 h DHA treatment for P. falciparum K1 trophozoites from microarray (1653 genes) and RNA-seq (2349 genes) experiments were compared. The overlap of down-regulated genes is shown in (a) and overlap of up-regulated genes in (b). The distributions of DHA-induced change in expression of differentially expressed genes with respect to average level of gene expression are shown for microarray in (c) and RNA-seq in (d). The log₂ (DHA/vehicle) values for 1653 genes from microarray and 2349 genes from RNA-seq were divided equally into 8 intervals of average expression. The distributions of log₂ (DHA/vehicle) values in each interval were determined by beanplot analysis; the mean gene expression change in each interval is shown by a horizontal line and the distributions of gene expression change are indicated by the “bean” shapes. Wilcoxon rank sum test of these distributions showed that 3/28 pairwise comparisons in the microarray data are significant (p < 0.05), whereas 21/28 pairwise comparisons in the RNA-seq data are significant (p < 0.05)