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Table 2 Differential protein species in paper mulberry leaves during cold treatment identified by MALDI-TOF/TOF MS

From: The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation

Spot no. Expression pattern 0, 6, 12, 24, 48, 72 (h) Accession no. Protein name Organism La Theor. Mr (kDa)/pI Exp. Mr (kDa)/pI Score SCb(%) NPc
Anti-oxidation and defense
6 BAD27393 Glutathione reductase Zinnia violacea CP 53.4/6.49 53/5.98 127 4 1
21 EXC33221 L-ascorbate peroxidase Morus notabilis CP 27.4/5.43 27/6.16 242 11 3
28 XP_004501840 Methionine sulfoxide reductase Cicer arietinum - 22.3/5.38 25/6.69 306 22 4
34 EXB55011 Peroxiredoxin Morus notabilis - 22.6/8.91 20/6.33 117 5 1
35 ABK22037 Peroxiredoxin Picea sitchensis C 25.7/9.11 16/4.67 187 12 2
38 EXB51369 Thioredoxin M4 Morus notabilis C 20.2/9.14 14/4.50 135 10 4
Calvin cycle
10 EXC10712 Rubisco activase Morus notabilis C 52.1/6.39 43/5.12 476 14 3
2 AAN63283 Rubisco LSU Broussonetia papyrifera C 51.5/5.83 65/4.91 264 12 2
4 AAN63283 Rubisco LSU Broussonetia papyrifera C 51.5/5.83 59/4.89 333 15 3
7 AAN63283 Rubisco LSU Broussonetia papyrifera C 51.5/5.83 51/6.41 598 20 8
3 P34794 RuBisCO LSU-binding protein Brassica napus C 61.7/5.14 61/4.85 144 4 1
Citrate cycle
8 AAB39989 Fumarase Arabidopsis thaliana M 53.4/7.98 50/6.99 239 8 3
15 EXC01795 Malate dehydrogenase Morus notabilis M 35.9/6.11 37/6.33 279 18 4
37 EXC35650 Nucleoside diphosphate kinase group1 Morus notabilis - 16.3/6.84 15/7.00 338 32 4
16 EXB51441 Solute inorganic pyrophosphatase Morus notabilis - 33.4/6.61 33/5.01 380 22 3
Glycolysis
5 XP_002267091 Enolase Vitis vinifera CP 48.3/6.17 58/6.06 230 8 1
12 EXC04020 GAPDH Morus notabilis CP 38.4/7.68 39/7.0 179 12 3
Photosynthesis
20 EXC20796 PsbP domain-containing protein 5 Morus notabilis C 30.5/7.72 28/6.94 162 11 2
33 XP_002300858 oxygen-evolving complex protein 2 Populus trichocarpa C 28.3/7.68 19/5.63 105 10 1
22 EXB93720 Plastid lipid-associated protein 6 Morus notabilis C 30.7/8.53 27/4.96 175 18 1
24 EXB93720 Plastid lipid-associated protein 6 Morus notabilis C 30.7/8.53 26/5.16 155 18 2
27 EXC34900 Chlorophyll a/b binding protein Morus notabilis C 28.5/5.46 25/4.79 92 9 1
30 EXB80094 Chlorophyll a/b binding protein Morus notabilis C 26.7/6.10 21/5.56 489 15 5
Protein processing
1 XP_007033710 heat shock protein 70 isoform 1 Theobroma cacao C 71.6/5.67 70/4.88 1094 18 7
18 EXB97663 Heat shock protein 90 Morus notabilis - 83.7/4.82 30/4.54 780 11 7
31 ACG38358 Prefoldin subunit 2 Zea mays - 16.3/5.84 20/5.28 136 19 1
13 XP_007223366 Proteasome subunit Prunus persica - 40.4/8.10 37/5.00 227 13 3
25 EXC32303 Proteasome subunit Morus notabilis - 33.7/8.83 26/6.20 713 32 8
9 EXC10716 Protein TIC 62 Morus notabilis C 51.1/7.68 43/4.90 153- 3 1
Transcription and Translation
23 EXB94487 Elongation factor Tu Morus notabilis C 52.2/6.30 38/5.09 108 3 1
11 EXB94487 Elongation factor Tu Morus notabilis C 52.2/6.30 39/5.12 397 15 4
14 EXB39373 mRNA-binding protein Morus notabilis C 44.5/7.11 38/6.41 292 12 4
19 EXC31412 Ribonucleoprotein Morus notabilis C 38.1/4.68 29/4.52 118 9 1
36 AEW08900 RNA binding protein Pinus taeda C 5.3/4.89 15/4.93 49 19 1
  1. aSubcellular location; C: chloroplast; CP: cytoplasm; M: mitochondria; −: no site information
  2. bProtein sequence coverage
  3. cNumber of matched peptides with significant scores identified by MALDI-TOF/TOF MS